rabbit anti leptin  (Santa Cruz Biotechnology)

Journal: Frontiers in Endocrinology

Article Title: Integrative bioinformatics analysis identifies placental senescence-associated signatures in early-onset preeclampsia

doi: 10.3389/fendo.2026.1863608

Figure Lengend Snippet: Experimental validation supports a senescence-associated role of LEP in EOPE. (A) qRT–PCR analysis of LEP, ENG, MIF and CYBB mRNA expression in PTB and EOPE placentas. n = 6 biologically independent placentas per group. Bars represent mean ± SD. (B) Representative images of senescence-associated β-galactosidase (SA-β-gal) staining. (C) Representative immunofluorescence images of P21 in PTB and EOPE placentas. Nuclei were counterstained with Hoechst. (D) Representative images of LEP immunohistochemistry in PTB and EOPE placentas. Scale bars, 100 μm. n = 6 biologically independent placentas per group. (E) Representative western blot showing LEP protein expression in PTB and EOPE placentas. n = 6. (F) Western blot analysis of P21 and LEP protein expression in BeWo cells treated with increasing concentrations of leptin. (G) Representative SA-β-gal staining images (left) and quantification of SA-β-gal-positive cells (right) in BeWo cells treated with leptin. n = 3 biologically independent experiments. Bars represent mean ± SD. (H) Detection of hCG in cell culture supernatant by ELISA after 24 h of leptin treatment.

Article Snippet: The obtained placental tissue sections were incubated overnight at 4 °C in a humidified incubation chamber with the primary antibody: anti-rabbit leptin (A1300, ABclonal).

Techniques: Biomarker Discovery, Quantitative RT-PCR, Expressing, Staining, Immunofluorescence, Immunohistochemistry, Western Blot, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: Materials Today Bio

Article Title: Reverse biogradient biomimetic periosteum with osteogenic and angiogenic characteristics for bone regeneration

doi: 10.1016/j.mtbio.2025.101967

Figure Lengend Snippet: The biomimetic periosteum increases PSCs accumulation in the bone defect region and upregulates periostin expression. (A) Immunofluorescence staining of PSCs (OSX + LepR + osteoprogenitors, yellow) in the bone defect region of the control, GC, GC-N, GC-NB and CM groups at 6 weeks. Scale bars, 500 μm. The higher-magnification images of the upper row (indicated by square boxes) are listed in the second row. Scale bars, 200 μm. (B) Immunofluorescence staining images of periostin in the femur defect at 6 weeks post-surgery. Scale bars, 100 μm. Data are presented as mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. P, Periosteum; C, cortical bone; DAPI, 4′,6-diamidino-2-phenylindole; OSX, Osterix; LepR, leptin receptor; GC, biomimetic periosteum without Func-HA and BMP-2; GC-N, biomimetic periosteum without Func-HA; GC-NB, biomimetic periosteum; CM, collagen membrane. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Furthermore, decalcified femoral sections were incubated with antibodies against CD31 (Abcam, 1:500), Osterix (OSX, Abcam, 1:500), periostin (Abcam, 1:500), α-smooth muscle actin (αSMA, Service, 1:200), endomucin (Emcn, Service, 1:200) and leptin receptor (LepR, ABclonal, 1:200) overnight at 4 °C, followed by incubation with biotinylated secondary antibodies or fluorophore-conjugated secondary antibodies in the dark.

Techniques: Expressing, Immunofluorescence, Staining, Control, Membrane

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Effects of different pre-conditioning exercise on leptin synthesis and its downstream signalling pathway in T2DM rats

doi: 10.22038/ijbms.2024.77774.16828

Figure Lengend Snippet: List of primary and secondary antibodies: sources and product models

Article Snippet: Rabbit anti-Leptin , Boster Biological Technology , BA1231.

Techniques: